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willysr
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Heinz Wiesinger
63daf9f79a
All: Support $PRINT_PACKAGE_NAME env var
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| README | 5c821121ec academic/perlprimer: Updated for version 1.2.3_f7d3bd0. | 6 rokov pred |
| References | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 rokov pred |
| doinst.sh | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 rokov pred |
| perlprimer.SlackBuild | 63daf9f79a All: Support $PRINT_PACKAGE_NAME env var | 3 rokov pred |
| perlprimer.desktop | 084a12139b academic/perlprimer: Make .desktop validate. | 7 rokov pred |
| perlprimer.info | 1c0f8805e8 academic/perlprimer: Updated for version 1.2.4. | 5 rokov pred |
| perlprimer.png | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 rokov pred |
| slack-desc | 5c821121ec academic/perlprimer: Updated for version 1.2.3_f7d3bd0. | 6 rokov pred |
README
PerlPrimer is a free, open-source GUI application written in Perl
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.
PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry
An optional run-time dependency is spidey, for Real Time PCR.
For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.
PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry
An optional run-time dependency is spidey, for Real Time PCR.
For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472