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- Bowtie is an ultrafast, memory-efficient short read aligner geared
- toward quickly aligning large sets of short DNA sequences (reads) to
- large genomes. It aligns 35-base-pair reads to the human genome at a
- rate of 25 million reads per hour on a typical workstation. Bowtie
- indexes the genome with a Burrows-Wheeler index to keep its memory
- footprint small: for the human genome, the index is typically about
- 2.2GB (for unpaired alignment) or 2.9GB (for paired-end or colorspace
- alignment). Multiple processors can be used simultaneously to achieve
- greater alignment speed. Bowtie can also output alignments in the
- standard SAM format, allowing Bowtie to interoperate with other tools
- supporting SAM, including the SAMtools consensus, SNP, and indel
- callers.
- If you use Bowtie for your published research, please cite:
- Langmead B, Trapnell C, Pop M, Salzberg SL.
- Ultrafast and memory-efficient alignment of short DNA sequences to
- the human genome. Genome Biol 10:R25.
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