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- Primer3 is a widely used program for designing PCR primers (PCR =
- "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool
- in genetics and molecular biology. Primer3 can design hybridization
- probes and sequencing primers.
- PCR is used for many different goals. Consequently, primer3 has many
- different input parameters that you control and that tell primer3
- exactly what characteristics make good primers for your goals.
- For details and citation:
- Steve Rozen and Helen J. Skaletsky (2000) Primer3 on the WWW for
- general users and for biologist programmers. In: Krawetz S, Misener S
- (eds) Bioinformatics Methods and Protocols: Methods in Molecular
- Biology. Humana Press, Totowa, NJ, pp 365-386
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