Дерево:
331a473f1a
11.0
12.0
12.1
12.2
13.0
13.1
13.37
14.0
14.1
14.2
15.0
bedlam
bedlam-name-fixes
dive
erik
gitignore
libressl-gopher-test
master
misc-fixes
python-renames
rworkman
urchlay
user/aclemons/updates
user/anddt/development/acpica
user/anddt/development/glm
user/anddt/development/gnustep-base
user/anddt/development/gnustep-make
user/anddt/games/assaultcube-reloaded
user/anddt/games/doomseeker
user/anddt/games/prboom-plus
user/anddt/games/rocksndiamonds
user/anddt/games/rottexpr
user/anddt/games/srb2
user/anddt/graphics/blender
user/anddt/graphics/maim
user/anddt/ham/chirp
user/anddt/ham/hamlib
user/anddt/libraries/girara
user/anddt/libraries/goffice
user/anddt/libraries/gtksourceview4
user/anddt/libraries/imlib2
user/anddt/libraries/jsoncpp
user/anddt/libraries/libnova
user/anddt/libraries/libopenmpt
user/anddt/libraries/libtorrent
user/anddt/libraries/wxGTK3
user/anddt/libraries/xmlrpc-c
user/anddt/misc/discount
user/anddt/misc/slop
user/anddt/network/nethogs
user/anddt/network/putty
user/anddt/network/rclone
user/anddt/network/rtorrent
user/anddt/network/sshguard
user/anddt/network/strongswan
user/anddt/network/umurmur
user/anddt/network/yt-dlp
user/anddt/network/znc
user/anddt/office/gnumeric
user/anddt/office/sigil
user/anddt/system/FontAwesome
user/anddt/system/conky
user/anddt/system/fakeroot
user/anddt/system/hplip-plugin
user/anddt/system/postgresql
user/anddt/system/testdisk
user/anddt/system/worker
user/anddt/system/xen
user/anddt/system/zzzfm
user/belka/updates
user/chris.willing/updates
user/dslackw/updates
user/elyk/updates
user/kingbeowulf/updates
user/mario/updates
user/ponce/updates
user/pprkut/updates
willysr
wxWidgets-wip
GPG-KEY
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Heinz Wiesinger
63daf9f79a
All: Support $PRINT_PACKAGE_NAME env var
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| README | 5c821121ec academic/perlprimer: Updated for version 1.2.3_f7d3bd0. | 6 лет назад |
| References | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 лет назад |
| doinst.sh | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 лет назад |
| perlprimer.SlackBuild | 63daf9f79a All: Support $PRINT_PACKAGE_NAME env var | 3 лет назад |
| perlprimer.desktop | 084a12139b academic/perlprimer: Make .desktop validate. | 7 лет назад |
| perlprimer.info | 1c0f8805e8 academic/perlprimer: Updated for version 1.2.4. | 5 лет назад |
| perlprimer.png | f82ed85b01 academic/perlprimer: Added (Design primers for PCR and sequencing) | 13 лет назад |
| slack-desc | 5c821121ec academic/perlprimer: Updated for version 1.2.3_f7d3bd0. | 6 лет назад |
README
PerlPrimer is a free, open-source GUI application written in Perl
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.
PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry
An optional run-time dependency is spidey, for Real Time PCR.
For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472
that designs primers for standard PCR, bisulphite PCR, real-time PCR
(QPCR) and sequencing. It aims to automate and simplify the process
of primer design.
PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both
sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local
server
- Results can be saved or optionally exported in a tab-delimited
format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted
to be in-frame
- QPCR primer design without manual intron-exon boundary entry
An optional run-time dependency is spidey, for Real Time PCR.
For details and citation:
Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
standard, bisulphite and real-time PCR. Bioinformatics 2004
20(15):2471-2472